Figure captions
Figure 1 . Van diagram with the iELISA and qPCR results
for the 451 serum samples analysed.
Figure 2. Seroprevalence in Iberian hares by year (A) and
season (B). Seroprevalence includes only results from hares randomly
sampled (hunted or live captured), showing the proportion of the sample
with antibodies recognizing myxoma virus antigens with 95% confidence
interval.
Figure 3. Dot graph with the RI10 values from serum
samples collected in different years. The left graph (A) refers to data
from serum samples collected from hunted or live trapped hares. The
right graph (B) refers to data from found dead hares’ serum samples. The
two horizontal lines point out the IR value, the lower line representing
the original cut-off of iELISA (IR=2.0) and upper line (IR 6.1)
indicates the adjusted ELISA cutoff value. M- macroscopic lesions
compatible with MYXV infection (no qPCR results available), N-negative
in qPCR, P-positive in qPCR, ND-no data available on the qPCR test.
Figure 4. Sample size and seropositivity by
administrative units in 1994-1999 and 2017-2019. A) Number of Iberian
hares sampled; B) Administrative units with seropositive Iberian hares,
considering the whole sample. Administrative units are districts in
Portugal and provinces in Spain.
Figure 5. Hypotheses for Iberian hare susceptibility to
MYXV and MYXV-related viruses. A-Hares are not susceptible to infection
by MYXV strains B-Hares are susceptible to MYXV if exposed to high
infectious doses; C- ha-MYXV emerged in hares after a species jump
event, involving or not, an intermediate host; D- Hares are infected by
MYXV, but develop subclinical disease; E-ha-MYXV emerged in hares
infected with rabbit strains, by a recombination event that increased
virulence of the virus towards hares; F-ha-MYXV emerged in hares from a
strain that has circulated unnoticed in the population.