Specific IgE measurements
Specific IgE measurements were performed with both ImmunoCAP™ Phadia 250
(Thermo Fisher Scientific, Phadia AB, Uppsala, Sweden) and NOVEOS
(Hycor, Garden Grove, CA, USA) systems, in full compliance with NF EN
ISO 15189 standards (certification #8-1769). Following the initial
determination by the ImmunoCAP method, samples were kept frozen at -40°C
before testing with NOVEOS. For statistical analyses, sIgE values
outside of the analyzers’ ranges of measurement (ImmunoCAP: 0.10 -
100kUA/L; NOVEOS: 0.17 - 100 kUA/L) were
adjusted to 0.10 or 0.17 kUA/L respectively for results
below these values, or to 100 kUA/L for results
>100 kUA/L. Some samples were tested after
addition of a CCD-blocker reagent (ProGlyCan MUXF3-human serum albumin,
Hämosan, Ilz, Austria) at a final concentration of 20 µg/mL.