Results analysis and statistics
Analytical correlations and general agreement between NOVEOS and ImmunoCAP were calculated by using the Spearman’s formula, Cohen‘s kappa index, and percentage of agreement (proportion of both true positive and true negative results). Clinical performance of specific IgE values was determined through odd-ratios (OR; Baptista-Pike’s confidence intervals) and relative risks (RR; C.I. according to Koopman’s asymptotic score) of presenting with allergy, and also through calculation of receiver operator curves (ROC), kappa index, and percentages of agreement. For these analyses, individuals were categorized for each allergen and technique into four groups: “true positives” (confirmed allergy and sIgE above the cut-off value for the relevant allergen), “true negatives” (confirmed tolerance and sIgE below the cut-off value for the relevant allergen), “false negative” (confirmed allergy and sIgE below the cut-off value for the relevant allergen), and “false positive” (confirmed tolerance and sIgE above the cut-off value). All statistical calculations were performed by using PRISM 9 (GraphPad Software, San Diego, California USA,www.graphpad.com). Significance was set at p>0.05. Optimal cut-off of sIgE values were calculated using Youden’s index (21).