3.3| Effects of carbonic anhydrase and Na+/H+ exchanger inhibitors
Increased expression of genes involved in Na+ uptake could be a possible cause of the death of H. tridens and M. japonicus in 513.3 mmol/L NaCl solution. To examine this possibility,H. tridens and M. japonicas were reared in 513.3 mmol/L NaCl solution supplemented with acetazolamide or amiloride, an inhibitor of CA and NHE, respectively. Amiloride significantly increased the survival rate of H. tridens and M. japonicus , and acetazolamide increased the survival rate of M. japonicus reared in 513.3 mmol/L NaCl solution (Fig. 6B–D). The survival rate was also higher in H. tridens reared in 513.3 mmol/L NaCl solution supplemented with acetazolamide compared to 513.3 mmol/L NaCl solution, but the results were not statistically significant (Fig. 6A).