2.7| Statistical analysis
All rearing experiments to evaluate survival rates were performed at
least in triplicate, and Na+, K+,
and osmotic concentrations were measured in at least three crabs in each
group. Error bars in the figures show standard errors. Mean values
comparisons among more than three groups were performed using analysis
of variance (ANOVA), followed by Williams’ test, Student’s t -test
with Bonferroni correction, or Tukey’s test. Mean values comparisons
between two groups were performed by Student’s t -test. Results
were considered statistically significant if the P value was less
than 0.05.