Low temperature Chl a fluorescence (77K)
Low temperature (77 K) Chl fluorescence emission spectra were measured
using a Perkin Elmer Luminescence Spectrometer (LS50B) (Buckinghamshire,
England) equipped with liquid nitrogen accessory. Algal cultures from
dark-adapted (10 min) mid-log phase cultures were transferred to NMR
tubes and flash frozen in liquid nitrogen. Fluorescence spectra were
collected at the excitation wavelength of 435 nm and recorded at a slit
width of 4 nm for excitation and emission. Decompositional analysis of
fluorescence emission spectra in terms of five Gaussian bands was
performed by a non-linear least squares algorithm according to
Morgan-Kiss et al. (2002b) using the program OriginPro 8.5.1.