2.11 Gus staining
Tobacco seedlings harboring the ProNtMYB12a : Gus vector
were transformed to MS medium containing 0, 50, 88 (Control), 150, and
200 mM sucrose for 7 days. Gus staining was performed with a GUS
Staining Kit (Coolaber, SL7160) following the manufacturer’s
instructions. The seedlings were immersed in the staining buffer
provided by the kit under vacuum infiltration for 30 min, and then kept
at 37°C overnight. The green seedlings were decolorized with 95% and
75% ethanol successively. The photographs were taken using an OLYMPUS
SZ 61 stereomicroscope.