The coding sequences and amino acid sequences of Arabidopsis AtMYB12
(At2g47460), AtMYB11 (At3g62610), and AtMYB111 (At5g49330) were used as
query sequences to make blast in the NCBI database. Six homologous
proteins were identified (XP_016480310.1, XP_016482665.1,
XP_016508470.1, XP_016487451.1, XP_016444804.1, and XP_009790561.1)
in the N. tabacum database, and the full length gene sequences
and coding sequences were amplified and sequenced with gene-specific
primers (Supplementary Table S1). Exon/intron structure of each gene was
determined by aligning the CDS and genomic DNA sequences with Clustal X
(version 1.83). Multiple sequence alignment of the NtMYB proteins was
performed using the DNAMAN (version 6.0) software with default gap
penalties (Jeanmougin, Thompson, Gouy,
Higgins, & Gibson, 1998). The phylogenetic tree was built up using the
MEGA 5.0 software (Tamura et al., 2011)
and the following protein accessions in GenBank: AtMYB90 (AAG42002),
AtMYB75 (AAG42001), AtMYB113 (NP_176811), PhAN2 (AAF66727,Petunia hybrid), MdMYB10 (XP_028963317.1, Malus
domestica), VvMYBA1 (BAD18977.1, Vitis vinifera), VvMYBA2
(BAD18978.1), MdMYB11 (AAZ20431.1), MdMYB12 (XP_008337875.1), GhMYB38
(AAK19618.1, Gossypium hirsutum), VvMYBPA2 (ACK56131.1), AtMYB123
(Q9FJA2), MdMYB9 (NP_001280749.1), PmMBF1 (AAA82943.1, Picea
mariana), MdMYB22 (AAZ20438.1), SlMYB12 (ACB46530, Solanum
lycopersicum), ZmP (P27898, Zea maize), GhMYB1 (CAD87007.1,Gerbera hybrid cultivar), LjMYB12 (BAF74782.1, Lotus
japonicas), and VvMYBF1 (FJ948477).