2.4 Subcellular localization of NtMYB12a-GFP fusion protein
The subcellular localization of NtMYB12a was analyzed in tobacco protoplasts as described previously (Yoo, Cho, & Sheen, 2007). The recombinant 35S:NtMYB12a-GFP and 35S: GFP plasmid was introduced into tobacco protoplasts obtained from leaf tissues using polyethylene glycol-mediated transformation. The fluorescent signal was captured 16-20 h after transformation with a confocal laser scanning microscope (ZEISS LSM 700, Germany). The nuclear localized marker protein (NLS) was as described in the previous reference (Shcherbo et al., 2007).