Healthy donors blood cells
Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coat
collected from Red Cross. Blood samples were diluted 1:2 with PBS/2 mM
EDTA, layered on a density gradient (Ficoll-Paque-Plus density gradient)
and centrifuged at 2000 rpm for 25 min. Interface containing PBMCs were
carefully removed and cells were washed twice with PBS/EDTA followed by
resuspension in RPMI1640 supplemented with 10% heat-inactivated foetal
bovine serum (FBS) and 1 % Penicillin/Steptomycin or cryopreservation
in FBS/DMSO for future analysis.