Supplementary Figures and Tables
Figure S1. Relative abundances of microbial community taxonomy based on annotation of contigs, represented at the phylum level. Contigs were constructed through IDBA-ud and annotated using the Integrated Microbial Genomes & Microbiomes (IMG/M) system.
Figure S2. Extended error plots for functional profiles regarding DNA repair, helicase and topoisomerase, protein biosynthesis, and transport and chemotaxis. Profiles were visualized through STAMP based on annotation of metagenomic reads using SEED subsystems. The pvalues are represented and were calculated using Fisher’s exact two-sided test, with the confidence intervals calculated by the method from Newcombe-Wilson. Samples are clustered and colored according to environmental temperature, following the three different groups: 98oC fumarole, <80 oC fumaroles and glaciers.
Figure S3. A circular view of the 158 metagenome-assembled genomes (MAGs) that were recovered through anvi’o v. 5 pipeline and are represented with the mean coverage of contigs, and the MAGs redundancy, completeness, GC content, total reads mapped and number of SNVs reported. The clustering dendrogram in the center shows the hierarchical clustering of contigs based on their sequence composition, and their distribution across samples.
Figure S4. Heatmap representing the 13 high quality and 82 medium quality MAGs based on read mapping per sample with Z-score. Samples are clustered and colored according to environmental temperature, following the three different groups: 98 oC fumarole, <80 oC fumaroles and glaciers.
Table S1. Physical-chemical parameters data per sample including temperature, pH, EC (electrical conductivity), B, Cu, Fe, Zn, OM (organic matter), OC (organic carbon), P, Si, Na, K, Ca, Mg, sulfate, nitrogen, ammonia, nitrate, sand, silt, and clay.
Table S2. General information about reads and contigs annotation.
Table S3. P -values of Spearman correlations comparing taxonomic and functional profiles with the environmental data.
Table S4. A complete list of all reconstructed MAGs with their characteristics: taxonomic classification based on GTDB-Tk and GhostKoala, the total genome length, N50, GC content, redundancy and completeness, based on anvi’o and CheckM, and the genome quality status.
TABLE 1