2.9 Adoptive transfer of MDSCs
For adoptive transfer, 2×106CD11b+Gr1+cells isolated from the
spleen of the IMQ-induced mice (wild type/WT and iNOS gene knockout/
iNOS-/-) via flow cytometry were washed twice and
resuspended in 200 μL of PBS and injected into mice via the tail vein as
shown in Figure 6A. Briefly, MDSCs were injected the day before the
application of IMQ and injected again on the third day after the
application of IMQ.