2.5 Isolation of human PBMCs
The work described has been conducted in accordance with The Code of
Ethics of the World Medical Association. Informed consent was obtained
from all recruited subjects. Both healthy control participants and
patients with psoriasis were subjected to peripheral blood drawing (6
mL/person), and human PBMCs were isolated by density-gradient
centrifugation using Ficoll-Paque (GE Healthcare Bio-science AB Uppsala,
Sweden). All plasma samples were stored at -80 °C for later analysis.
The PBMCs at a concentration of 1 × 106/mL were
maintained in RPMI 1640 (Thermo Fisher Scientific) culture medium
supplemented with 10 % FBS; Thermo Fisher Scientific).