2.9 Adoptive transfer of MDSCs
For adoptive transfer, 2×106CD11b+Gr1+cells isolated from the spleen of the IMQ-induced mice (wild type/WT and iNOS gene knockout/ iNOS-/-) via flow cytometry were washed twice and resuspended in 200 μL of PBS and injected into mice via the tail vein as shown in Figure 6A. Briefly, MDSCs were injected the day before the application of IMQ and injected again on the third day after the application of IMQ.