Additional experiments evaluating N and P allocation in mycelia
Stoichiometric data derived from nutrient gradient tests suggested a
differential allocation of N and P in fungal mycelia during growth. To
test such patterns of element allocation, data of fungal strains growing
on SEA and glucose media were analyzed in this context.
12 fungal strains (RLCS01, RLCS16, RLCS27, RLCS15, RLCS13, RLCS28,
RLCS22, RLCS17, RLCS09, RLCS18, RLCS12, RLCS11; n=3) were grown on SEA,
analyzing N contents after 12 and 26 days. Additionally, the N content
of inner and outer mycelium of four strains (RLCS12, RLCS16, RLCS22,
RLCS28; n=3) was analyzed after 26 days of growth. Therefore, the radius
of fungal mycelia at four positions was determined, and partitioned by
2/3 (inner) and 1/3 (outer). Mycelia were cut and divided into inner and
outer parts according to this separation, and element contents analyzed
separately.
Similarly, in an additional nutrient manipulation experiment the inner
and outer mycelium of fungi grown in glucose media with high N (C:N 20)
and low N content (C:N 200) were harvested separately using the same
mycelial partitioning. Four fungal strains were selected based on large
variation of 1/HCN values see below), also using fungal
strains previously characterized in another study (RLCS06, RLCS18,
RLCS09, RLCS13; unpublished data).