Microchannel dialysis method
The denatured CA solution was fed from the feed channel inlet of the microchannel in Fig. 3 at a rate of 0.05 mL/min (τ = 20 min), and 0.05 M Tris-HCl refolding buffer was fed from the inlet of the permeation channel at a rate of 0.5 mL/min. The solution was collected from the outlet of the feed-side channel for each 30 minutes, and the amount of collected samples, CA concentrations, and enzyme activities were measured.