Figure legends
Figure 1. Schematic of permeation cell for the measurement of the permeation coefficient of GdnHCl through a dialysis membrane.
Figure 2. Schematic of microchannel dialysis setup. Feed and permeation flow channel were set in counter current.
Figure 3. Design of microchannel dialysis setup; GdnHCl solution with/without enzyme was fed from feed flow channel (PDMS) inlet and pure water/refolding buffer was fed from permeation flow channel (PMMA) inlet.
Figure 4. Time course of GdnHCl concentration in permeation experiment. Inset represents the magnification of first 100 min.
Figure 5. Time course of GdnHCl concentration in microchannel permeation. Feed flow rate was 0.1 mL/min (τ = 10 min) in 0–50 min, 0.05 mL/min (20 min) in 50–150 min, 0.025 mL/min (30 min) in 150–300 min.
Figure 6. (a) CA concentration after refolding (CA concentration before refolding is 0.25 mg/mL), (b) recovered soluble CA after refolding. CA amount (CA concentration [mg/mL] × solution amount [mL]) before refolding is set as 100%, (c) relative activity of CA after refolding. CA esterase activity normalized by CA concentration was compared. (d) total activity of CA after refolding, which is the product of protein recovery and relative activity.