Figure legends
Figure 1. Schematic of permeation cell for the measurement of the
permeation coefficient of GdnHCl through a dialysis membrane.
Figure 2. Schematic of microchannel dialysis setup. Feed and permeation
flow channel were set in counter current.
Figure 3. Design of microchannel dialysis setup; GdnHCl solution
with/without enzyme was fed from feed flow channel (PDMS) inlet and pure
water/refolding buffer was fed from permeation flow channel (PMMA)
inlet.
Figure 4. Time course of GdnHCl concentration in permeation experiment.
Inset represents the magnification of first 100 min.
Figure 5. Time course of GdnHCl concentration in microchannel
permeation. Feed flow rate was 0.1 mL/min (τ = 10 min) in 0–50
min, 0.05 mL/min (20 min) in 50–150 min, 0.025 mL/min (30 min) in
150–300 min.
Figure 6. (a) CA concentration after refolding (CA concentration before
refolding is 0.25 mg/mL), (b) recovered soluble CA after refolding. CA
amount (CA concentration [mg/mL] × solution amount [mL]) before
refolding is set as 100%, (c) relative activity of CA after refolding.
CA esterase activity normalized by CA concentration was compared. (d)
total activity of CA after refolding, which is the product of protein
recovery and relative activity.