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\begin{document}

\title{Prevalence and phylogenetic characterizations of Enterovirus D68 strains
among respiratory infection cases in Beijing, 2018-2019}



\author[1]{Aihua Li}%
\author[2]{Tiegang zhang}%
\author[3]{Cheng Gong}%
\author[2]{Maozhong Li}%
\author[3]{Fang Huang}%
\affil[1]{Beijing Center for Disease Prevention and Control}%
\affil[2]{Affiliation not available}%
\affil[3]{Beijing Center for Disease Prevention and Control, Beijing Preventive Medicine Research Center}%


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  \date{May 22, 2024}


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\begin{abstract}
Backdround: Enteroviruse-D68 (EV-D68) has been endemic in Beijing for
some years. This study analyz the prevalence and phylogenetic
characterizations of EV-D68 in Beijing during 2018 to 2019. Methods: The
clinical specimens were collected from respiratory infection cases of 30
sentinel hospitals in Beijing and subjected to EV and EV-D68 detection
by real-time PCR. Results: Of 15, 645 respiratory infection cases,
467(2.98\%) cases were enterovirus positive and 14(0.09\%) were EV-D68
positive. The detection rates of EV-D68 were 0.11\% (9/7, 837) in 2018,
0.06\% (5/7, 805) in 2019, respectively. Of these EV-D68 infection
patients, 7 cases were aged younger than 18 years, 3 were aged 18 to 60
years and 4 aged over 60 years. 4 cases (28.6\%) were diagnosed as upper
respiratory tract infection and 10 (71.4\%) as pneumonia, including one
with severe pnuemonia. The main symptoms for EV-D68 patients were fever
(10/14,71.4\%) and cough (9/14, 64.3\%). 7 cases were detected in summer
and the rest cases in the autumn. The phylogenetic analysis suggested
that EV-D68 strains from 2018 belonged to a major subclade B3 and a
minor subclade D3, while all strains in 2019 were clustered into
subclade D3. These subclade B3 or subclade D3 strains were located in a
separated evolutionary branches,respectively. Conclusions: Our research
further elucidate the continuous molecular evolution and genetic
variability of EV-D68 occured in Beijing, China.%
\end{abstract}%



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\textbf{Hosted file}

\verb`Prevalence and phylogenetic characterizations of Enterovirus D68 strains among respiratory infection cases in Beijing, 2018-2019.doc`  available at \url{https://authorea.com/users/348837/articles/474037-prevalence-and-phylogenetic-characterizations-of-enterovirus-d68-strains-among-respiratory-infection-cases-in-beijing-2018-2019}

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