16S rRNA amplicon Library Preparation
Illumina MiSeq platform was used to perform 16S rRNA amplicon sequencing using the protocol described by Caporaso et al in 201079 . Briefly, we used universal primers 341F and 805R to amplify V3-V4 hypervariable region of 16S rRNA gene. The target amplified product size was approximately 464 bp. The V3­V4 primer and the adapter details are as mentioned below.
V3­V4 amplification primers