Functional Metagenome & Pathway Analysis
For functional analysis, MOCAT2 was used to cluster high quality reads
into reference gene catalogs which is then annotated to Evolutionary
genealogy of genes: non-supervised orthologus group (eggnog)
database46 . The gene abundance profile was
calculated for each sample by mapping the raw reads back to the
reference gene catalogue. Functional mapping and Analysis Pipeline
(FMAP)47 , was used to compare the various
time points for alteration in pathways, using the Kruskal-Wallis
rank-sum test.