Preparation of allogenic RBCs from donor rabbits
Donor rabbits (n =16) were anaesthetised via intravenous injection
of 25 mg/kg ketamine and 10 mg/kg xylazine. As described previously, 50
mL/kg of donor blood was withdrawn from the femoral
artery17. After removing the platelet-rich plasma and
platelet-poor plasma (PPP) from the blood via centrifugation (100
×g for 15 min), the remaining RBCs were washed with acid citrate
dextrose solution. Then, the same volume of mannitol adenine phosphate
solution (1.457% [w/v%] D-mannitol, 0.014% adenine and 0.094%
sodium dihydrogen phosphate [Terumo, Tokyo, Japan]) was added to
prepare allogenic RBCs that were then stored at 4°C. PPP samples showed
the following coagulation activity: fibrinogen, 121 mg/dL; AT III
activity, 81%; prothrombin time (PT), 13.1 sec; activated partial
thromboplastin time (APTT), 31.2 sec, on average.