Analyses of plasma nitric oxide
Plasma
NO2-/NO3-(nitric oxide, NOx) levels were measured every 15 min.
Blood samples were collected in heparin and centrifuged at 50,000
×g at 4°C for 20 min to remove HbV particles. The supernatant was
stored at -80℃ until analysis. Plasma NOx levels were
measured at the Eicom Laboratory (Eicom Corporation, Kyoto, Japan) using
a high-performance liquid chromatography-Griess system, as previously
described22.