Overexpression of STAT3 relieves GGC-promoted apoptosis and reduction in viability.
In addition, to confirm whether the mitigation of STAT3 activation was crucial in GGC-driven apoptosis, A549 cells were transfected with a STAT3C-expressing construct followed by GGC exposure. As shown Fig. 2H, STAT3C-overexpression remarkably increased p-STAT3(Tyr705) and STAT3 levels. Moreover, this increase led to a substantial decrease in GGC-regulated apoptosis and its effect on viability as compared with control cells (Fig. 1F and Fig.4F).