Overexpression of STAT3 relieves GGC-promoted apoptosis and
reduction in viability.
In addition, to confirm whether the mitigation of STAT3 activation was
crucial in GGC-driven apoptosis, A549 cells were transfected with a
STAT3C-expressing construct followed by GGC exposure. As shown Fig. 2H,
STAT3C-overexpression remarkably increased p-STAT3(Tyr705) and STAT3
levels. Moreover, this increase led to a substantial decrease in
GGC-regulated apoptosis and its effect on viability as compared with
control cells (Fig. 1F and Fig.4F).