Figure 6. NMR spectra zoomed in at the signal of IVM (left) comparison with metabolite M1 (middle) and M3 (right).
Structure of the IVM was labeled in yellow at signals showing most significant differences in the spectra of the metabolites. The cursor in HSQC spectrum indicates signals missing due to changes in the metabolites. Panel A) the shift of the –CH group in the metabolite M1 (m/z 883) at the position labeled in yellow and B) the missing –CH3 group of the metabolite M3 (m/z 913) at position labeled in yellow. The changes in chemical shift values are listed in Table 3.
Figure 7. Proposed metabolic pathway of ivermectin metabolites.