Histopathological Investigation
For histopathological investigation healthy and diseases infected fourth instar larvae were collected from Regional Tasar Research Institute, Manipur, India. Different organs like silk glands and gut were removed from normal and nucleopolyhedrovirus (NPV) infected silkworms. The removed tissues were preserved in 10% formaldehyde solution. The tissues were again fixed in Bouin’s fluid. The water molecules were removed with the help of alcohol and embedded in paraffin wax for sectioning. 5-7µm thick tissues were stained with haematoxylin and eosin. Structural examination and histopathological changes were identified by visualization through Leica DM 3000 LED and photographed with Leica DFC450 C.