Sequence analysis
Online similarity searches were performed using the BLAST algorithm of
GenBank. Sequence alignment was carried out with the CLUSTALW program by
MEGA7 software(S. Kumar, Stecher, & Tamura, 2016). The phylogenetic
analyses based on the 16S rDNA gene for proteolytic bacteria were
further used to establish relationships among them. The partial 16S rDNA
sequences obtained for were utilized in the search of reference
nucleotide sequence available in NCBI GenBank database using BlastN
algorithm to retrieve the similar sequences(Altschul et al., 1997).
Phylogenetic tree and evolutionary relationships of bacterial isolates
was constructed by Neighbor-Joining method (Saitou & Nei, 1987) with
Kimura 2-parameter corrections (Kimura, 1980). To calculate the support
for each clade, bootstrap analysis were performed with 1000 replications
(Felsenstein, 1985).