Histopathological Investigation
For histopathological investigation healthy and diseases infected fourth
instar larvae were collected from Regional Tasar Research Institute,
Manipur, India. Different organs like silk glands and gut were removed
from normal and nucleopolyhedrovirus (NPV) infected silkworms. The
removed tissues were preserved in 10% formaldehyde solution. The
tissues were again fixed in Bouin’s fluid. The water molecules were
removed with the help of alcohol and embedded in paraffin wax for
sectioning. 5-7µm thick tissues were stained with haematoxylin and
eosin. Structural examination and histopathological changes were
identified by visualization through Leica DM 3000 LED and photographed
with Leica DFC450 C.