Figure legends
Figure 1. Inhaled rosuvastatin reversed the decreases in the expression and activity of brainstem CD73 in OVA-sensitized rats. The expression of CD73 in NA-containing brain tissue was assayed with Western-blot; and the activity was measured with molecular biological methods. Note in OVA-sensitized rats, both the expression and activity of CD73 were significantly decreased, leading to significantly increased ATP concentration in cerebral spinal fluid, and all of which were reversed by inhaled rosuvastatin in rosuvastatin-treated rats. P values were obtained from one-way ANOVA.
Figure 2. Inhaled rosuvastatin abolished the increase of airway vagal activity in OVA-sensitized rats. (A1, B1 and C1) The bar graphs of inspiratory resistance of the airway (Ri), expiratory resistance of the airway (Re) and dynamic compliance of the lungs (Cdyn) measured using plethysmography, showing that in OVA-sensitized rats, Riand Re were significantly increased; Cdyn was significantly decreased, and all of which were reversed by inhaled rosuvastatin in rosuvastatin-treated rats. (A2, B2 and C2) The bar graphs of Ri, Re and Cdyn 20 min after intraperitoneal injection of atropine sulphate (0.5 mg·kg-1), showing that the changes induced by OVA sensitization were abolished. (A3, B3 and C3) comparison of Ri, Re and Cdynbefore and after atropine treatment in rats of different groups, showing that atropine significantly decreased Ri and Re, and significantly increased Cdyn in OVA-sensitized rats, but did not cause any change in Ri, Re and Cdyn of control and rosuvastatin-treated rats. P values in the left and middle columns were obtained from one-way ANOVA; P values in the right column was obtained from paired t -test.
Figure 3. Inhaled rosuvastatin reversed the increase in the expression of ECP1 in the lungs of OVA-sensitized rats . P values were obtained from one-way ANOVA.