2.2 Plant materials and temperature treatment
The high-quality rice (Oryza sativa L. ) variety Wuyujing 3 (W3)
was used as the plant material for its sensitivity of the quality
formation to the increased temperature and the large area of cultivation
in the experimental region. In order to evaluate the individual and
co-impact effects of the increased temperature, field treatments were
conducted as follows: (i) normal temperature (CK); (ii) elevated
temperature (ET). Temperature treatment was carried out with the
free-air temperature enhancement system (FATE) from full heading (80%
of the rice in the field starts the heading stage). Each treatment was
designed with 3 replications and 80cm intervals and protection lines
were set up between the treatment blocks to ensure the independence of
the experiment. Rice cultivation methods were adopted by seedling
raising and artificial transplanting. Field management measures
including fertilization, wet-dry alternate irrigation, prevention of
pests and weeds were carried out according to the local high-yielding
cultivation measures.