2.4 UV photoactivation
The recording chamber of the Port-a-Patch was customized to accommodate
a 400 µm diameter quartz optic fiber, which was placed 3–4 mm (or 5-7
mm in a subset of experiments) above the recorded cell. The original
perfusion manifold was replaced by a custom manifold positioned to the
side of the recording chamber opposite to the waste removal. Solution
exchange was complete within 1–2 s. UV light pulses were delivered by a
310 nm fiber-coupled Mightex FCS-0310-000 LED (Mightex, Pleasanton, CA),
with 40 µW intensity. Pulses were triggered by the PatchMaster software.