2.7 Statistics
The data and statistical analysis comply with the recommendations of the
British Journal of Pharmacology on experimental design and analysis in
pharmacology (Curtis et al., 2018). All values are given as mean ± SEM;
n is the number of cells recorded from, we collected no technical
replicates. Experiments with low seal resistance (< 1 GΩ),
high series resistance (> 10 MΩ), or small currents
(< 1 nA) were excluded from analysis. Because seal resistance
or series resistance occasionally changed during experiments, group
sizes varied between n = 6 and n = 9. This presents no problem in
statistical evaluation, since all post-treatment measurements were
compared to pre-treatment measurements in the same cell, therefore group
were of equal sizes in all statistical tests. The two-tailed, paired
Student’s t-test was used for comparing drug effects to control (in
Microsoft Excel). Normalization was done after statistical evaluation,
only for the sake of making observed effects comparable. In statistical
tests we chose to give the exact level of significance (value ofP ) rather than considering changes significant if P is
less than an arbitrary value (0.05), because this gives more information
regarding the magnitude and consistency of observed changes.