Description of inoculums prepared for soil spiking.
In experiments 1-3, whole blood was collected from wild boar
experimentally infected with ASFV “Armenia08”. These animal trials
were previously conducted for pathogenesis studies. The blood was mixed
for 15-20 minutes with glass beads to remove fibrin. The blood was then
stored at -80°C until use. Since experiments were completed at different
time points, stocks for spiking the soil matrices had different titers,
but differed no more than one log in considered volumes. Infected blood
from experiment 1 had a titer of 7.25 log10 50%
hemadsorbing doses (HAD50) per mL, blood from experiment
2 had a titer of 6.00 log10 HAD50/mL and
blood from experiment 3 had a titer of 7.00 log10HAD50/mL.
ASFV-Kenya1033ΔCD2vdsRed was derived from ASFV-Kenya1033 as described by
Hübner et al. (2018) by substitution of the CD2v ORF (EP402R) from codon
77 to the translational stop codon (386) by a dsRed expression cassette.