2.10. Statistics
All results are expressed as the mean ± SEM. Differences between means were assessed for statistical significance with a one-way analysis of variance (ANOVA) and post-hoc least significance tests. Differences between proportions were examined with the chi-squared test. All statistical analyses were performed with the GraphPad 8 software package (GraphPad Software, Inc., La Jolla, CA, USA), with statistical significance set at p< 0.05 .