*New genotype proposed
Figure 1. Identification of the porcine RVH positive samples by
the visualization under UV light of the RedSafe staining 2% agarose gel
electrophoresis of the amplification of a 1240 bp fragment of the RVH
VP6 gene. The pooled positive samples are identified as VC9, VC18, VC19,
VC29, VC32, VC36, VC39 VC41 and VC52 and the negative control as NEG.
Fig 2. Maximum Likelihood trees constructed with the Tamura-Nei model
for the VP7, VP4 and VP6 RVH segments. Numbers along the tree represents
the confidence value for a given internal branch based on 500 Bootstrap
replicates, only values larger than 70 are shown. The symbols (filled
circles) above the strains indicate the porcine Spanish RVH strains
identified in this study. GenBank accession number, country and year of
collection of fecal sample are also shown below the strains. Genotypes
are indicated on the right of the bracket. Scale bars indicate
nucleotide substitutions per site.