For the quantitative analysis for the performance of the proposed AgNMPA MEF substrate, the actual fluorescence intensities of the substrates were calculated from the fluorescence images. A circular indicator was assigned to each spot and the mean value of the intensity in circular indicator (Imean) was defined as the fluorescence intensity. The mean value of the intensity outside the circular indicator was defined as the background signal, which were commonly used definition in the fluorescence microarray analysis. Figure 7(a) shows the fluorescence intensity and background signal of AgNMPA MEF substrates with the deposition angles of 81°, 85°, and 89°. The average fluorescence intensity and standard deviation were calculated using all 150 spots in each substrate (5 x 5 spot array and 6 array on a substrate). The fluorescence intensity decreased as the deposition angle increased because the number of Ag nanorods decreased as increasing deposition angle. However, the background signal decreased more dramatically due to the enhancement of shadowing effects in higher deposition angle. Figure 7(b) compares the SBR ratio of each AgNMPA MEF substrate. It clearly shows that the maximum SBR was obtained from the AgNMPA MEF substrate with deposition angle of 89°. The SBR increased from 15.6 to 42 as the deposition angle increased.