Figure 5. Overlap in population and rearing expression differences across evolutionary lineages. Of the transcripts differentially expressed based on population of origin (top), 124 were overlapping between drainages. Of these, 66 were concordantly differentially expressed (i.e. expression divergence in the same direction in both drainages; dark orange circles) and 58 were not (i.e. expression divergence in opposite directions between drainages; light orange circles), no different than expected by chance. Of the transcripts differentially expressed based on rearing environment (bottom), 35 were overlapping in the two drainages. Of these, 19 were concordantly differentially expressed (dark yellow circles) and six were not (light yellow circles), more non-concordance than expected by chance. The number of differentially expressed transcripts in each quadrant is indicated on the graphs and transcripts differentially expressed in one but not both drainages are shown in grey.