2.2. Biochemical assays
Fasting blood samples was taken at the beginning and at the end of the intervention. The serum and plasma were separated by centrifugation at 3000 rpm for 10 min. The serum samples were stored at −70 °C quickly after centrifugation until analysis. Serum leptin level was determined by Enzyme-linked immunosorbent assay kits following the manufacturer’s protocols (LDN Co., Nordhorn, Germany) and adiponectin (Booster Biological Technology Co., Wuhan, China).