2.2. Biochemical assays
Fasting blood samples was taken at the beginning and at the end of the
intervention. The serum and plasma were separated by centrifugation at
3000 rpm for 10 min. The serum samples were stored at −70 °C quickly
after centrifugation until analysis. Serum leptin level was determined
by Enzyme-linked immunosorbent assay kits following the manufacturer’s
protocols (LDN Co., Nordhorn, Germany) and adiponectin (Booster
Biological Technology Co., Wuhan, China).