Fine Needle Aspiration, Cytomorphological and
Mitotic-Karyorrhexis Index (MKI) evaluation
Fine needle aspiration cytology (FNAC) was performed by
palpation/image-guidance from various sites and all smears and
corresponding cell blocks with immunocytochemistry (if performed) were
retrieved and re-evaluated by two expert cytopathologists (RS, MR). Cell
blocks from FNA were prepared by plasma thromboplastin technique. These
were categorized further as per previously published INPC criteria
extended to cytopathology as undifferentiated, poorly differentiated,
differentiating neuroblastoma or
ganglioneuroblastoma.16 Mitotic-karyorrhexis index
(MKI) assessment was performed on well spread H&E-stained smears and
their corresponding cell blocks using digital image visual assessment
(DIVA).17