Automatic Detection and Classification of Ca2+ Release Events in Confocal Line- and Framescan Images
Recently, a method was developed by Tian et al., (Tian 2012), where fluorescence time trace in each pixel is fitted and provides a practically noise free approximation of the original fluorescence data. This pixel-by-pixel method had, however, several limitations which made it impractical to be used for Ca2+ release event detection.
Here we extend the method by Tian et al., in several ways. The new method allows for Ca2+ release event classification based on pixel-by-pixel denoising of the original signal.
Data acquisition was performed on two confocal setups. Linescan images were obtained on a Olympus FluoView 1000 confocal microscope. Framescan images were recorded with a VTInfinity multi-beam confocal microscope recording 512x64 pixel images at 150Hz freqency.
The detection algorithm is presented in the Results section.