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viii. Figure Legends:
Figure 1. (a) Photosynthetic capacity (i.e., maximal light- and
CO2-saturated rate of oxygen evolution) per leaf area,
(b) leaf dry mass per area, (c) level of chlorophyll a + bper leaf area, and (d) chlorophyll a/b ratio in leaves of IT (red
columns) and SW (blue columns) plants that were grown in low light/warm
temperature growth conditions (LLW), low light/cool temperature growth
conditions (LLC), high light/warm temperature growth conditions (HLW),
or high light/cool temperature growth conditions (HLC). Mean values ±
standard deviations (n = 3 or 4); groups that share the same
letters are not considered statistically different, and groups that do
not share the same letters are considered statistically different based
on one-way ANOVA and post hoc Tukey–Kramer HSD tests.
Figure 2. Relative transcript abundance of (a) CBF1 , (b)CBF2 , and (c) CBF3 in leaves of IT (red columns) and SW
(blue columns) plants that were grown in LLW, LLC, HLW, or HLC. Values
are presented relative to the expression level for each respective gene
in the IT ecotype grown under LLW. Mean values ± standard deviations
(n = 3); groups that share the same letters are not considered
statistically different, and groups that do not share the same letters
are considered statistically different based on one-way ANOVA and post
hoc Tukey–Kramer HSD tests.
Figure 3. (a) Hierarchical clustering of the
log2 expression data for 7,933 genes with an adjustedP- value below 0.01 in one of the pairwise comparisons for
differential expression between ecotypes and growth conditions. The
three biological replicates for each growth condition/ecotype set are
shown as separate columns. (b-d) Log2 expression data
for IT and SW in HLC relative to LLW for (b) the subset of genes
involved in light reactions of photosynthesis that were downregulated in
IT under HLC, (c) the subset of genes involved in cyclic electron flow
around PSI, Calvin-Benson-Bassham cycle, and chlorophyll biogenesis that
were found to be induced in SW under HLC, and (d) CBF-regulated genes.
Figure 4. Reduction state of the primary electron acceptor of
photosystem II, QA, quantified by chlorophyll
fluorescence using the equation 1 − qL, for IT (red
circles) and SW (blue squares) in (a) LLW and (b) HLC. Mean values ±
standard deviations (n = 3); statistically significant
differences between ecotypes based on Student’s t -tests are
indicated with asterisks (* = P < 0.05, ** =P < 0.01, *** = P < 0.001);n.s . = not significantly different.
Figure 5. Cellular electrolyte leakage following exposures to
freezing temperatures of IT (red circles), it:cbf123 (light red
circles), SW (blue squares), sw:cbf2 (lighter blue squares), and
sw:cbf123 (lightest blue squares) in (a) LLW or (b) HLC, as well
as (c) images of leaves following exposures to freezing temperatures
with false colors based on photosystem II photochemical efficiency (as
Fv/Fm) for HLC plants. For (a) and (b),
mean values ± standard deviations (n = 3).
Figure 6. Transcript abundance for (a) CIPK25, (b)COR78, (c) LTI30, (d) COR15a, and (e) GolS3in leaves of IT (red columns), it:cbf123 (light red columns), SW
(blue columns), sw:cbf2 (lighter blue columns), and
sw:cbf123 (lightest blue columns) plants that were grown in the
LLW or HLC conditions. All values are normalized based on the expression
levels of IT in LLW. Mean values ± standard deviations (n = 3);
groups that share the same letters are not considered statistically
different, and groups that do not share the same letters are considered
statistically different based on one-way ANOVA and post-hoc
Tukey–Kramer HSD tests.
Figure 7. (a) Photosynthetic capacity (i.e., light- and
CO2-saturated rate of oxygen evolution) per leaf area,
(b) leaf dry mass per area, (c) level of chlorophyll a + bper leaf area, and (d) chlorophyll a/b ratio in leaves of IT (red
columns), it:cbf123 (light red columns), SW (blue columns),
sw:cbf2 (lighter blue columns), and sw:cbf123 (lightest
blue columns) plants that were grown in LLW or HLC. Mean values ±
standard deviations (n = 3 to 5); Groups that share the same
letters are not considered statistically different, and groups that do
not share the same letters are considered statistically different based
on one-way ANOVA and post-hoc Tukey–Kramer HSD tests.
Figure 8. (a) Leaf thickness of IT (red column),
it:cbf123 (light red column), SW (blue column), sw:cbf2(lighter blue column), and sw:cbf123 (lightest blue column)
plants that were grown in HLC, as well as representative images of leaf
cross-sections for (b) IT, (c) it:cbf123 , (d) SW, and (e)
sw:cbf123 . For (a), mean values ± standard deviations (n =
3); groups that share the same letters are not considered statistically
different, and groups that do not share the same letters are considered
statistically different based on one-way ANOVA and post-hoc
Tukey–Kramer HSD tests.
Figure 9. (a) Rosette diameter of IT (red column),
it:cbf123 (light red column), SW (blue column), sw:cbf2(lighter blue column), and sw:cbf123 (lightest blue column) after
40 days of growth in HLC, as well as images of representative (b) IT,
(c) it:cbf123 , (d) SW, and (e) sw:cbf123 plants. For (a),
mean values ± standard deviations (n = 5); groups that share the
same letters are not considered statistically different, and groups that
do not share the same letters are considered statistically different
based on one-way ANOVA and post-hoc Tukey–Kramer HSD tests.
Figure 10. Relative transcript abundance for (a) SUS1,(b) EGR2, (c) RCI2A, (d) AT5G44565, (e)AT1G13930 , and (f) LCR69 in leaves of IT (red columns),
it:cbf123 (light red columns), SW (blue columns), and
sw:cbf123 (light blue columns) plants grown in LLW or HLC. All
values are normalized based on the expression levels of IT in LLW. Mean
values ± standard deviations (n = 3); groups that share the same
letters are not considered statistically different, and groups that do
not share the same letters are considered statistically different based
on one-way ANOVA and post-hoc Tukey–Kramer HSD tests.