Fungal taxonomic composition
The
C1 and C2 samples of control plots were not saturated in sequence depths
(less than 10,000 sequences, based on the rarefaction curves in Fig.
S1), probably due to technical
problems of DNA sequencing. Therefore, they were discarded for data
analyses. After resampling of raw
28S rRNA gene reads, a total of 126,050 high-quality sequence reads were
generated and were clustered into 991 OTUs at the 97% identity.Ascomycota (70.66±11.80%)
was the dominant phylum, followed by Basidiomycota(14.21±10.19%), Chytridiomycota (0.51±0.64%) andBlastocladiomycota (< 0.01%). Those OTUs were assigned
to 135 genera and the most abundant genera included Kriegeria(9.14±9.64%), Hyaloscypha (6.63±5.41%), Collophora(3.43±6.50%), Mollisia (2.20±3.68%), Russula(0.80±3.40%), Sorocybe (0.57±1.77%), Rigidoporus(0.46±1.53%), Chloroscypha (0.45±0.78%), Hymenoscyphus(0.41±0.61%) and Crinula (0.40±1.00%).
The fungal genera were further
classified into 6 ecological groups based on their roles in ecosystems
(Tedersoo et al., 2014) - saprotrophs (57%), plant pathogens (24%),
ectomycorrhizal fungi (6%), animal parasites (5%), mycoparasites (2%)
and mycobionts (6%), among a total of the 135 genera. However, soil
warming did not change the overall fungal community composition,
reputing part of our first hypothesis (Table 1 & Fig. S2a).
Additionally, no differences in
diversity and environmental group
abundances were found between warmed and control samples (Table S3).