To understand the effects of the natural environment on the pigment and antioxidant profiles, we compared lab-cultured S. caninervis plants to those collected from the field. Shoots from a previously isolated clone of a S. caninervisherbarium specimen from southern Nevada, U.S.A. (Stark NV-107, USA, Nevada, Clark County, Newberry Mts, Christmas Tree Pass; UNLV) were cultivated in a growth chamber set to an 18-hour photoperiod (18 °C light, 12 °C dark), at ~30 µmol m-2 s-1 PAR. Cultures of a single genotype were grown from fragments in lidded 77 m × 77 m × 97 m Magenta GA-7 plant culture boxes (bioWORLD, Dublin, OH, USA) on 1.2% agar made with an inorganic nutrient solution (Hoagland and Arnon, 1950).