To explore potential mechanisms of UV tolerance, transcript abundance was compared in field UV-filtered and UV-transmitted plants. Samples were collected in June 2019 from six window pairs (those that had sufficient tissue remaining after previous analyses) and stored dry, at room temperature in the dark until processing in July 2020. Stems were selected from each window sample and dead tissue and debris were removed. Approximately 20 mg of dry weight per sample were placed into microcentrifuge tubes and sent to Novogene (Novogene, Sacramento, CA, USA) for RNA extraction, library preparation, and transcriptome sequencing. Samples were processed according to standard Novogene protocol, including preliminary quality checks gel electrophoresis, quantitation, and purity assessment with NanoDrop (ThermoFisher Scientific, Waltham, MA, USA), and sample integrity assays with a Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA). After quality checking procedures, oligo(dT) beads were used to enrich eukaryotic mRNA and rRNA was removed with the Illumina Ribo-Zero kit (Illumina, Inc., San Diego, CA, USA). RNA samples were then reverse-transcribed into double-stranded cDNA libraries and sequenced on the 150 bp paired-end Illumina NovaSeq 6000 platform.