Genotyping of ESBL-producing and COL-resistance genes
For further molecular characterization, 95 isolates were randomly selected among 325 ESBL-producing E. coli isolates. Genotyping of these isolates by PCR assay showed that among ESBL determinants, blaCTX-M was the most common (n=86), followed by blaTEM (n=32) and blaSHV (n=30). While in COL-resistant genes, only mcr-1 was identified and prevalent (n=75) in these isolates. The blaTEM and blaSHV genes were most commonly detected in combination with blaCTX-M, and mcr-1 Figure 2A.