MLST and population structure
A total of 22 isolates were organized into a total of 20 sequence types (STs) with seven groups and 15 edges. Sites from where the strains were got, antibiotic resistance genes, plasmid replicon, STs, and antibiogram are stated in Table 5. Moreover, STs found along with the allelic profile of 22 typeable E. coli isolates are given in Table 6.
The total epidemiological facts of the dominant STs were based on the MLST data of the selected isolates (n=22) revealed a total of 20 STs. Sequence types 58 and ST410 were the most predominant, comprising two each (2/20=20%). All other STs were present found only once. Details of distribution and abundance of STs are labeled in Figure 3A and 3B. The most prevalent STs (ST58) and (ST410) were originated from Zhejiang and Jiangsu, respectively.
All 20 STs originated in this study were grouped into 7 BURST group and 17 based on goeBURST analysis. Interestingly, no double locus variants (DLV) were identified among all 20STs; however, a total of 4 single locus variants (SLV) were identified among all the seven groups. The major BG (BG 0) contained six isolates with 6 STs (ST354, ST2737, ST2113, ST4355, ST3901, and ST3736) with a predicted founder of ST354 (Figure 3.3.C) comprised of 3 SLVs. A second BG consisted of 5 isolates and 5 STs (ST58, ST2108, ST4024, ST865, and ST3599) with ST58 as predicted founder Figure 3.3.D. The burst analysis with all 7256 STs accessible in the database, including all 20 STs mentioned in this report, revealed that the majority of the STs were founders such as ST10 and ST58 Figure 3C, 3D, and 3E. Unpredictably, geoBURST analysis revealed that the majority of the STs found in the isolates under study (ST58, ST10, ST410, and ST178, etc.) were all founders, while other such as ST2108 and ST2113 were delineated as co-founders.