Overexpressing miR-210 inhibited autophagy in hypoxic H9C2
cells
Next, the expression of autophagy-associated proteins LC3 and p62 were
assessed by western blot to investigate the effects of miR-210
overexpression on autophagy in hypoxic H9C2 cells. As shown in Figure 3,
LC3 II/I was upregulated and p62 was downregulated in the hypoxia group
compared with the normoxia group, which demonstrated that there was a
certain degree of autophagy in H9C2 cells. Furthermore, the expression
of LC3 II/I was significantly lower, and the expression of p62 was
higher in the miR-210 mimic group compared with other groups, which
indicated that miR-210 overexpression inhibited
autophagy
in H9C2 cells under hypoxic conditions.