The expression of miR-210 in H9C2 was upregulated in hypoxic
H9C2 cells.
To generate an optimized model of
anoxic myocardial cells, H9C2 cells were treated with
CoCl2 for 24, 48 and 72 h. The number of apoptotic cells
and relative miR-210 expression are shown in Figure 1. With increasing
CoCl2 concentrations and treatment duration, the
apoptosis ratio of H9C2 cells was enhanced (Figure 1A). Additionally,
the relative expression of miR-210 increased in a time-dependent manner
(Figure 1B). We found that a 24 h and 48 h hypoxia treatment provoked
similar apoptosis ratios in H9C2 cells. Therefore, a 48 h hypoxia
duration was used in H9C2 cardiomyocytes for all subsequent experiments.