Overexpressing miR-210 inhibited cell apoptosis in hypoxic H9C2 cells
We evaluated the effects of miR-210 overexpression on cell apoptosis in hypoxic cardiomyocytes by generating NC, miR-210 mimic and miR-210 inhibitor groups under hypoxia culture for 48 h. The relative expression of miR-210 was potently enhanced in the miR-210 mimic group but was suppressed in the miR-210 inhibitor group, which demonstrated the high transfection efficiency in H9C2 cells (Figure 2A). Moreover, there was no significant difference in the apoptosis ratio between hypoxia and NC groups (data not shown). Overexpressing miR-210 caused a decline in apoptosis, whereas silencing miR-210 had the opposite effect. It was uncovered that overexpressing miR-210 inhibited cell apoptosis in hypoxic H9C2 cells (Figure 2B and C).