Figure legends
Figure. 1 Effects of CoCl2 on hypoxic myocardial cells. A. Effects of CoCl2 concentration and treatment duration on the apoptotic ratio of H9C2 cells; B. Effects of different hypoxic treatment times on the relative miR-210 expression.
Figure. 2 Effects of miR-210 on cell apoptosis in hypoxic cardiomyocytes. A. Transfection efficiency in NC, miR-210 mimic and miR-210 inhibitor groups; B. Effects of miR-210 mimic and miR-210 inhibitor groups on H9C2 cell apoptosis; C. H9C2 cell apoptosis was analysed by flow cytometry.
Figure. 3 Effects of miR-210 on autophagy in hypoxic cardiomyocytes. A. Expression of LC3I and LC3II proteins in the five groups; B. The grey value analysis of LC3 II/I expression in H9C2 cells; C. Expression of p62 proteins in H9C2 cells; D. The grey value analysis of p62 (of β-actin) in the five groups. LC3, light chain 3; NC, negative control.
Figure. 4Caspase8ap2 is a putative target of miR-210 in H9C2 cells. A. The putative target site of miR-210 was highly conserved in the Caspase8ap2 mRNA 3’UTR; B. Caspase8ap2 3’UTR along with a plasmid encoding miR-210; **, P< 0.01.
Figure. 5 Effects of miR-210 on Caspase8ap2 signalling pathway.A. Expression of Caspase8ap2, caspase 8, caspase 3 and Beclin-1 proteins determined by western blot; B. Effects of exogenous miR-210 expression on the protein level of Caspase8ap2; C. Effects of miR-210 overexpression on caspase 8 and caspase 3 protein levels; D. Effects of exogenous miR-210 on the protein level of Beclin-1; NC, negative control.