Overexpressing miR-210 inhibited autophagy in hypoxic H9C2 cells
Next, the expression of autophagy-associated proteins LC3 and p62 were assessed by western blot to investigate the effects of miR-210 overexpression on autophagy in hypoxic H9C2 cells. As shown in Figure 3, LC3 II/I was upregulated and p62 was downregulated in the hypoxia group compared with the normoxia group, which demonstrated that there was a certain degree of autophagy in H9C2 cells. Furthermore, the expression of LC3 II/I was significantly lower, and the expression of p62 was higher in the miR-210 mimic group compared with other groups, which indicated that miR-210 overexpression inhibited autophagy in H9C2 cells under hypoxic conditions.