Caspase8ap2 was a target gene of miR-210
To explore whether Caspase8ap2 was a
target gene of miR-210, we performed a dual-luciferase reporter gene
assay in H9C2 cells. Our computational studies identified consensus
putative target sites of miR-210 with high complementarity relevant to
the 3’UTR region of Caspase8ap2 (Figure 4A). For the luciferase reporter
assay, a dual-luciferase construct was generated. Remarkably decreased
luciferase activity was observed on pEZX-miR-201 co-transfecting with
the pEZX-Luc-Caspase8ap2 3’UTR (Figure 4B; P < 0.01).
These results showed that miR-210 could inhibit the activity of
luciferase on the Caspase8ap2 3’UTR reporter gene cattier.