miR-210 mediated apoptosis and autophagy of H9C2 cells via targeted-regulating of Caspase8ap2
To further investigate the mechanism of miR-210 in regulating Caspase8ap2 and its associated signalling pathway, we determined the effects of exogenous miR-210 on the expression of Caspase8ap2 and downstream associated proteins by western blot (Figure 5A). Beclin-1 was upregulated in the hypoxia group compared with the normoxia group. Protein levels of Caspase8ap2, cleaved caspase 8 and cleaved caspase 3 were decreased in response to overexpressing miR-210 (Figure 5B and C). Beclin-1, which regulates both apoptosis and autophagy, was also downregulated in the miR-210 mimic group (Figure 5D). On the other hand, the expression of Caspase8ap2 and other proteins in response to silencing miR-210 were increased. Therefore, miR-210 likely mediates apoptosis and autophagy in H9C2 cells via targeted-regulation of Caspase8ap2.