miR-210 mediated apoptosis and autophagy of H9C2 cells via
targeted-regulating of Caspase8ap2
To further investigate the mechanism of miR-210 in regulating
Caspase8ap2 and its associated signalling pathway, we determined the
effects of exogenous miR-210 on the expression of Caspase8ap2 and
downstream associated proteins by western blot (Figure 5A). Beclin-1 was
upregulated in the hypoxia group compared with the normoxia group.
Protein levels of Caspase8ap2, cleaved caspase 8 and cleaved caspase 3
were decreased in response to overexpressing miR-210 (Figure 5B and C).
Beclin-1, which regulates both apoptosis and autophagy, was also
downregulated in the miR-210 mimic group (Figure 5D). On the other hand,
the expression of Caspase8ap2 and other proteins in response to
silencing miR-210 were increased. Therefore, miR-210 likely mediates
apoptosis and autophagy in H9C2 cells via targeted-regulation of
Caspase8ap2.