Figure 3: Histochemical study of decellularization process.
[E-a] HE stained in control showed well-organized blue nucleus chondrocytes resides in lacunae and preserved ECM.
[A] HE stained decellularized ear pinna in [M1-M5] methods showed no cells. M2, M3, M5 method showed fast decellularizaion as compared to other, while at 80th cycle no any nucleus showed.
[E-b] DAPI staining in native ear pinna showed prominent blue fluorescents nucleus.
[B] DAPI staining in decellularized scaffold showed no nucleus. Method 5, M2, and M3 showed complete decellularization earlier than other methods.
[E-c] Massiontrichrome staining in native showed a well-organized form of ECM blue color collagen and elastin.
[C] The organization and mass of collagen fibers remain unaffected after decellularization in all methods.
[E-d]AB pH 2.5 staining in native ear pinna.
[D]AB pH 2.5 staining confirmed that the GAG has been preserved and its organization appeared to remain unaffected after decellularization