Figure 7: Histochemistry of scaffold after transplantation.
[A] HE stained in grafted ear pinna showed recruited chondrocytes ECM and chondrocytes with its lacunae.
[B] Masson’s trichrome staining shows a columnar type of chondrocyte in most places and has an abundant collagen matrix. The red coloration of the hyaline cartilage, reflecting proteoglycan content, is homogenous and the surface of the cartilage is smooth.
[C] AB pH 2.5 staining confirmed that the GAG organization. [D] Toluidine blue staining confirmed in grafted ear pinna chondrogenic differentiation.