Figure 7: Histochemistry of scaffold after transplantation.
[A] HE stained in grafted ear pinna showed recruited chondrocytes
ECM and chondrocytes with its lacunae.
[B] Masson’s trichrome staining shows a columnar type of chondrocyte
in most places and has an abundant collagen matrix. The red coloration
of the hyaline cartilage, reflecting proteoglycan content, is homogenous
and the surface of the cartilage is smooth.
[C] AB pH 2.5 staining confirmed that the GAG organization. [D]
Toluidine blue staining confirmed in grafted ear pinna chondrogenic
differentiation.