3. RESULTS
3.1. Isolation and culture expansion ofsuprapatellar fat pad
derived mesenchymal stem cells
Cells from SPFP samples were isolated by enzymatic digestion. After
cultures had been incubated for 3 days, the isolated cells markedly
spread out and adhered to the plastic culture flask. By 4-5 days, cells
exhibited homogenous spindle-shaped, fibroblast-like morphology. As time
went on, the cells evinced a high proliferation rapidly and took on a
clustered appearance. Cultures reached 80–90% confluence in 7 -10
days. Following the first sub-culturing, approximately 4-5 days were
needed for each passage. Cell morphology remained homogeneous until
being terminated (Fig. 1).