3.2. Effect of Andrographolide on the cell viability of
differentiating suprapatellar fat pad derived mesenchymal stem cells
To investigate the effect of AG on cell viability, the MTT assay was
performed by measuring mitochondrial activity. The result showed that
the OD values of SPFP-MSCs were stable following treatment with AG which
did not influence cell viability when it was used at concentrations
between 1.56 and 12.5 µM, indicating that cytotoxicity was undetectable
when the concentration of AG was less than 12.5 µM. However, AG
demonstrated the ability to decrease cell viability significantly at
dosages above 12.5 µM, suggesting that high concentrations of
andrographolide could inhibit cell proliferation of SPFP-MSCs (Fig. 2).