Relationship between the Microbiome, Metabolic Syndrome, and Chronic Inflammation
Next we compared features of the microbiome and subject clinical characteristics, including components of metabolic syndrome as well as several markers of inflammation, amongst survivors. We did not observe a statistically significant correlation with either Shannon or Simpson Index and Hemoglobin A1c, High Density Lipoprotein, Low Density Lipoprotein, Adiponectin- Leptin ratio, systolic blood pressure, diastolic blood pressure, or body mass index (Supplementary Table 1). We also did not observe a significant correlation between Shannon or Simpson index and levels of C-Reactive protein, Tumor Necrosis Factor alpha, or Interleukin-10 (Supplementary Table 2). A modest correlation was seen between Simpson index and levels of Interleukin-6 amongst survivors (R=-0.41, P=0.02), but no correlation was seen between Shannon index and Interleukin-6 levels (R=-0.04, P=0.81).
We also examined differently abundant taxa in subjects based on adiposity, body mass index and inflammation. Subjects with less favorable Adiponectin-Leptin ratio (<1.0) had increased abundance of multiple Bacteroides ASVs (Supplemental Figure 3) . Survivors with differing body mass index and C-Reactive protein levels also demonstrated differently abundant taxa after adjusting for a FDR of 0.05 (Supplemental Figure 4, Supplemental Figure 5) .