RPL26 gene annotation and verifying differential expression in CRSwNP by PCR
Through functional analysis of the core network as well as ribosome signaling pathway anchoring genes and NCBI database annotation we found that all the 15 key genes in the ribosome signaling pathway were included in the 16 core network interacting genes. There were 3 up-regulated genes and 13 down-regulated genes(Fig.3C). Through NCBI database and GenCards website notes, we found that RPL26 gene played an important role in this process. To verify whether RPL26 was differentially expressed in CRSwNP, we cultured nasal polyp tissue cells in vitro again. Normal nasal mucosa group (Control), nasal polyp group (CON) and dexamethasone intervention group (DTSG) were cultured for 3 days. All the cells were collected for quantitative PCR analysis. The results indicated that the RPL26 gene expression in nasal polyp tissues was lower normal nasal mucosa, but after dexamethasone intervention, the RPL26 gene expression level was significantly increased(Fig.4).