Construction of RPL26 silencing and overexpression cell model
Both RPL26 silencing and RPL26 overexpression lentivirus transfection plasmids were constructed with the help of the basic medical laboratory of Tongji University Medical School. CRSwNP cells at logarithmic growth stage were randomly divided into three groups, and then performed RPL26 gene silencing and overexpression transfection: Group A was transfected with blank plasmid(Control); Group B was transfected with RPL26 silencing plasmid(anti-RPL26); Group B was transfected with RPL26 overexpression plasmid(pre-RPL26). The cells were amplified for 3 days.