2.3. Serological evaluation
Serum samples were evaluated for T. gondii IgG with IBL-America Toxoplasma IgG enzyme-linked immunosorbent assay (ELISA) kit (Minneapolis, MN, USA) following the manufacturers’ instructions, for which the sensitivity and specificity values were more than 98%.T. gondii IgG titers were read at optical density 450 nm, and seropositivity was defined as a T. gondii IgG titer equal to or higher than 9 IU/mL.