S. cerevisiae yeasts were obtained from alcohol factory’s
effluent
A total of 52 microorganism samples from alcohol factory’s effluent
after preparation and cultivation of these samples on the complete
medium for yeast growth, were isolated and their total DNA were
extracted. Then the PCR method was performed to examine Zrt1 and Fet4
genes in these samples. Four samples expressing Zrt1 and Fet4 genes
which illustrated fragment patterns of 2102 bp and 165 bp on 1.5%
agarose gel in relation with Zrt1 and Fet4 genes, respectively, were
identified (figure 1A). Subsequently, after isolation of samples which
express Zrt1 and Fet4 genes, the yeast strains of these four samples
were identified using PCR of ITS1 and ITS4 primers. Then PCR products
were electrophoresed and results observed on 1.5% agarose gel and three
out of four samples illustrated fragment patterns in relation with ITS,
representing these three samples as yeast strains (figure1B). Then
related fragments were verified by sequencing and the results identified
two samples as S. cerevisiae yeasts (figure 1C). All results of
gel electrophoresis as well as phylogenetic tree of sequenced samples
were illustrated in figure 1 (to observe the results of sample
sequencing see the supplementary pdf file 1).