Determination of optimum pH for maximum zinc absorbed group and
examination of growth rate as well as Zrt1 and Fet4 expression level in
this pH
After determination of absorbed zinc by yeast biomass in all zinc
treated groups, a yeast biomass with maximum growth rate, absorbed zinc,
and expression level of Zrt1 and Fet4 in yeast cells, were selected.
This group was considered to study at pH 3, 4, 5 and 6, under conditions
that mentioned in the previous section, to find the optimum pH for
maximum zinc absorption. In the following, estimation of growth rate and
total accumulation of zinc in yeast cells as well as determination of
Zrt1 and Fet4 expression level in zinc absorbed yeasts were performed as
mentioned earlier (spectrophotometry, CDW estimation, and qRT-PCR).