Zinc concentrations change Zrt1 and Fet4 expression level inS. cerevisiae
The significant growth rate changes as well as maximum uptake of zinc by yeast cells at 24 h after inoculation was observed. Therefore, the qRT-PCR technique was applied to determine Zrt1 and Fet4 expression level in S. cerevisiae at 24 hours after inoculation. The results showed that under supplementing the SDB medium with different concentrations of zinc, the expression of Zrt1 and Fet4 changed noticeably. The Zrt1 expression reached a maximum level already after 24 h of incubation in 25 µg/ml of zinc concentration; also increment of Fet4 expression in present of 25 µg/ml of zinc was considerable. While the maximum level of Fet4 expression was observed in 50 µg/ml of zinc concentration. Hence, this relative quantification of Zrt1 and Fet4 expression verified the 24 h incubation and 25 µg/ml of zinc concentration as optimum time and zinc concentration, respectively forS. cerevisiae yeast (figure 3).
.