RESULT AND DISCUSSION
The results of this study showed leaf anatomical features ofHabenaria Wild Species in Shimogga district, comparable data among the species supports species identification and to helps to understand their structures adaption to different habitat in a district. The leaf anatomical structures showed organizations of components forming leaves, consisted of stomata, cuticle, epidermis, mesophyll and vascular bundles, hypodermis in cells are particular in orchid species.
1.Habenaria heyneana Lindl. : Leaf transverse section ofH . heyneana showed tetracyclic type of stomata, cuticle was thick and smooth, The epidermic was uniseriate with elongated shaped cells, hypodermis was not clear. Mesophyll were homogeneous consisted of 10-12 thin walled , round-shaped ,parenchymatic cell layers, filled with chlorophylls (chlorenchyms) vascular bundles consisted of xylem and phloem, arranged collaterally. There were 4 large and 4-6 small arches arranged in a row with parenchyma cells bundle sheath surrounded the vascular bundles, scherenchyma cells in vascular bundle were not observed ( figure 2. A , B. figure 4. I )
2. Habenaria Plantaginea Lind1. : H.Plantaginea anatomical characters showed that stomata was cyclocytic, cuticle was relatively thick and smooth. The epidermis was a single layer composed of elongated shaped cells . mesophyll was 13 -15 layers, consisted of elongated parenchymatous cells, chlorophylls were present in the Parenchymatous cells of mesophyll. vascular bundles were arranged within parenchymatous cells of mesophyll layer. Vascular bundles consisted of 7-9 Arches of xylem and phloem. Bundles sheath was thin walled surrounds the vascular bundles ( Figure 2. F , G,H. Figure 4. A)
3. Habenaria longicorniculata J.Graham. : H. longicorniculata anemocytic and tetracytic stomata configuration, cuticle layer was thick and smooth, epidermic was uniseriate composed of polygonal shaped cells. Hypodermis was not observed. Mesophyll was 14-15 layers of homogenous polygonal shaped parenchymatous cells. Vascular bundles consisted of xylem and phloem arranged collaterally. Sclerenchyma cells were not associated with vascular bundles, bundle sheath cells were thin . there were 8-9 arches of xylem and phloem arranged in a row embedded within parenchymatous cells of mesophyll. (figure 2 I, G Figure .4 B)
4. Habenaria elwessi Hook.f : H. elwessi had anemocytic stomata configuration and cuticle was thick and smooth, Epidermis is had one layer made up of elongated shaped cells . Hypodermis was absent or not clear. Mesophyll was Homogenous with 15-16 layers consisting of polygonal shaped parenchymatous cells. Chlorophylls were present in mesophyll cells. Vascular bundles consisted of xylem and phloem arranged collaterally. There were 9-10 xylem and phloem arches arranged in rows embedded in parenchymatous cells. Vascular bundles are surrounded with thin bundle sheath. ( Figure 3 , A, B, C figure 4. J).
5. Habenaria crinifera Lind1 : H. crinifera had tetracyclic stomata configuration with thick and smooth cuticle. Epidermis is single layered with elongated cells. Hypodermis was not clearly distinguishable. Mesophyll layer is made with Homogenous parenchymatous cells. Mesophyll cells stores starch grains and water. There is a large water storage cells were present. Mesophyll cells with 12-13 layers of parenchymatous cells, they were polygonal in shape. Vascular bundles with 10-11 xylem and phloem arches arranged in a rows embedded within parenchymatous cells. (Figure 3. D, E figure 4. H)
6. Habenaria ovalifolia Wight.: H. ovalifolia had tetracyclic stomata configuration with thick cuticle layer. The epidermis was uniseriate with polygonal shaped cells. Hypodermis is not clearly seen. Mesophyll was 14-15 layers with homogenous polygonal shaped parenchyma cells. Vascular bundles consisted of xylem and phloem arranged collaterally. Sclerenchyma cells not associated with vascular bundles. Bundle sheath was indistinct. There were 5-6 arches of vascular bundle arranged in a number of rows embedded within parenchymatous cells of mesophyll. (figure 3. F, G figure 4.F, G).
7. Habenaria multicaudata Sedgw. : H.multicaudata leaves has cyclocytic stomata configuration. cuticle was thick ,epidermis was a single layer with elongated cells. Hypodermis cells were not clear. Mesophyll was 13-14 layers made with homogenous , rounded to elongated shaped parenchyma cells. Chlorophylls were abundantly present in mesophyll cells. Vascular bundles were arranged with 4-5 arches. sclerenchyma cells were not associated with bundle sheath cells. Bundle sheath cells are thin cleanly visible. (figure 2 D, E figure 4.D).
8. Habenaria pelorioides Par. & Rchb.f. (Odisha cleistantha S.Mishra) : Leaf transverse section of H. peloriodes showed the stomata with anemocytic and tetracyclic configuration. Cuticle was thick and smooth in nature. The epidermis was uniseriate with elongated shaped cells. Hypodermis was absent or not clear. Mesophyll was homogenous consisted of 14-15 layers with thin walled parenchyma cells. Vascular bundles consisted of xylem and phloem arranged collaterally. There were 4-5 Arches arranged in a rows embedded with parenchyma cells. Sclerenchyma cells were not associated with vascular bundles. Thin walled bundle sheath surrounded the vascular bundle. (figure 3. H. I. figure 4. C,E ).