Screening of differentially expressed metabolic genes related to
survival
We obtained a total of 398 COAD samples and 39 normal colon samples from
TCGA for gene expression analysis and prognostic analysis, and
downloaded a dataset containing 1048 COAD samples (GSE40976) from the
GEO database. Downloaded the KEGG gene set c2.cp.kegg.v7.0.symbols.gmt
containing metabolic genes from the GSEA website, and extracted a total
of 921 Metabolism-related genes. Then, 645 cross-expressed genes were
screened in TCGA-COAD and GSE40976. P Value <0.05 and
|log2-fold change|> 0.5 were defined as
DEG. A heat map analysis was performed to show cluster analysis of gene
characteristics (Figure 1A), and a volcano map was constructed to reveal
225 significantly DEG (Figure 1B). A total of 377 cases were extracted
from TCGA-COAD, and 556 cases were extracted from GSE40976 for
prognostic analysis. Delete missing data and cases with survival time
less than 30 days from the cases. Univariate Cox regression analysis
revealed 22 PRMG in TCGA-COAD (Fig. 1C, p value <0.05).