PCR amplification of duplication´s breakpoints
The specific PCR amplification for the tandem duplication 5’ and 3’
breakpoints were based on the hypothesis of head-to-tail segmental
fusion and adjusted by WGS results (Supplementary table 1). Reactions
were performed following the enzyme’s manufacturer recommendations
[Promega, Madison, United States] in a thermal cycler [Biometra,
Germany]. Products were characterized by Sanger Sequencing.