Deletion Long Range-PCR (LR-PCR)
LR-PCR primers were designed according to inferred 5’ and 3’ breakpoints from SNP-array (Supplementary table 1). Amplification products ranged from 2.0 to 3.5kb. Reactions were performed following the enzyme’s manufacturer recommendations [KAPA Biosystem, Wilmington, United States] in a thermal cycler [Hangzhou Bioer Technology, China]. PCR products were analyzed by Sanger sequencing.