Human Androgen Receptor X-Chromosome Inactivation Assay (AR-assay)
The X-chromosome inactivation pattern (XIP) was performed on II1 and II3 by a methylation-based PCR of the androgen receptor (AR Xq12) first exon, as described by Allen et al(Allen et al., 1992).AR allele profiles and areas under the curve were obtained from capillary electrophoresis analysis of PCR products using GeneMarker V2.2.0 software [Softgenetics, State College, Pennsylvania].