Deletion Long Range-PCR (LR-PCR)
LR-PCR primers were designed according to inferred 5’ and 3’ breakpoints
from SNP-array (Supplementary table 1). Amplification products ranged
from 2.0 to 3.5kb. Reactions were performed following the enzyme’s
manufacturer recommendations [KAPA Biosystem, Wilmington, United
States] in a thermal cycler [Hangzhou Bioer Technology, China].
PCR products were analyzed by Sanger sequencing.