PCR amplification of duplication´s breakpoints
The specific PCR amplification for the tandem duplication 5’ and 3’ breakpoints were based on the hypothesis of head-to-tail segmental fusion and adjusted by WGS results (Supplementary table 1). Reactions were performed following the enzyme’s manufacturer recommendations [Promega, Madison, United States] in a thermal cycler [Biometra, Germany]. Products were characterized by Sanger Sequencing.