Multiplex Ligation-dependent Probe Amplification (MLPA)
MLPA kit for DMD (Salsas PO34-PO35) was used to find
deletions/duplications
(Gatta et al.,
2005; Janssen et al., 2005; Schwartz & Dunø, 2004). Reactions were
performed according to the manufacturer’s recommendations
[MRC-Holland, Amsterdam, The Netherlands (www.mlpa.com)]. Products
were analyzed using a fragment analyzer sequencer [ABI 3730XL; Applied
Biosystems, Foster City, California] and 500Liz as internal size
standard. Data analysis was performed using Coffalyser [MRC-Holland,
Amsterdam, The Netherlands] and GeneMarker V2.2.0 [Softgenetics,
State College, Pennsylvania] softwares. Wildtype, deleted, and
duplicated controls were included.