Effect of ABA on gametogenesis and gamete survival under salt
stress, long term experiment
Cells were diluted to ~ 1 ×
105 cells*mL-1 and were grown
vegetatively to a concentration of
~ 1 × 106 cells*mL-1,
then were centrifuged at 1500 g for 10 min and washed twice with
N-deficient (‒N) TAP medium before resuspension in 10% N medium.
Cultures were then treated with ABA or ethanol before adding 125 mM NaCl
as described above and were shaken-incubated at 200 rpm under continuous
illumination of 150 μmol photons m−2s−1 without dark periods, and monitoring the optical
density every 24 h for 72 h.