Effect of ABA on gametogenesis and gamete survival under salt stress, long term experiment
Cells were diluted to ~ 1 × 105 cells*mL-1 and were grown vegetatively to a concentration of ~ 1 × 106 cells*mL-1, then were centrifuged at 1500 g for 10 min and washed twice with N-deficient (‒N) TAP medium before resuspension in 10% N medium. Cultures were then treated with ABA or ethanol before adding 125 mM NaCl as described above and were shaken-incubated at 200 rpm under continuous illumination of 150 μmol photons m−2s−1 without dark periods, and monitoring the optical density every 24 h for 72 h.