Microscope imaging
H2DCFDA stained cells were visualized by a fluorescence microscope Leica SP8 confocal microscope equipped with Olympus DP10 digital camera, fitted with a 100 W High Pressure Mercury Burner with a lateral resolution down to 140 nm. Using CUDA based processing, differential interference contrast (DIC), chloroplast autofluorescence (excitation at 633 nm and emission at 670 nm) and DCF-DA epifluorescence (excitation: 488 nm, emission: 525 nm) images were obtained at 20× magnification.