Microscope imaging
H2DCFDA stained cells were visualized by a fluorescence
microscope Leica SP8 confocal microscope equipped with Olympus DP10
digital camera, fitted with a 100 W High Pressure Mercury Burner with a
lateral resolution down to 140 nm. Using CUDA based processing,
differential interference contrast (DIC), chloroplast autofluorescence
(excitation at 633 nm and emission at 670 nm) and DCF-DA epifluorescence
(excitation: 488 nm, emission: 525 nm) images were obtained at 20×
magnification.