Strain, Culture Conditions, and Growth Monitoring
Chlamydomonas reinhardtii strain CC-124 (mt- [137C]) was
generously obtained from laboratory of Dr. Iftach Yacoby, Tel Aviv
University, which was originally purchased from Chlamydomonas Resource
Center, University of Minnesota. The seed cultures were grown at 23 °C
on liquid Tris-acetate-phosphate (TAP) medium (Harris, 1989) in flasks
capped with a silicone sponge, and were shaken at 100 rpm under
continuous illumination of 150 μmol photons m−2s−1 at 16:8 h of light:dark period, and ambient
CO2 concentrations. Cell growth was determined by
optical density measurements at 750 nm (OD750) using a
NanoDrop 2000c spectrophotometer (Thermo Scientific, Wilmington, DE).
Cell count was performed with a BD FACSCalibur flow cytometer (Breda,
Netherlands). Cultures were kept in the log phase with
OD750 between 0.1 – 0.3 which is corresponding to a
cell number of 3 – 9 × 105cells*mL-1 by dilution every 48 h to minimize
self-shading.