Determination of salt stress
Exponential cultures of C. reinhardtii were inoculated onto TAP medium with elevated amounts of NaCl to final concentrations of 0, 100, 125, 175, 200, 250 and 300 mM, with initial OD750 of 0.1. The cultures were incubated at optimal conditions, in triplicates, and growth response was measured after 72 h of treatment.