Gene expression analysis
Total RNA was extracted from the grains or young panicle using the
RNAprep Pure Plant Kit (Tiangen). The HiScript II Q Select RT SuperMix
(Vazyme) was used for cDNA synthesis. The transcript levels of each gene
were measured by qRT-PCR using a 7500 Real-Time PCR System (ABI) with
PowerUp™ SYBR® Green Master Mix (Thermo Fisher Scientific). Gene
expression was quantified during the logarithmic phase using the
expression of the housekeeping gene Ubq (LOC_Os03g13170) as an internal
control. Three biological replicates were performed for each experiment.