Gene expression analysis
Total RNA was extracted from the grains or young panicle using the RNAprep Pure Plant Kit (Tiangen). The HiScript II Q Select RT SuperMix (Vazyme) was used for cDNA synthesis. The transcript levels of each gene were measured by qRT-PCR using a 7500 Real-Time PCR System (ABI) with PowerUp™ SYBR® Green Master Mix (Thermo Fisher Scientific). Gene expression was quantified during the logarithmic phase using the expression of the housekeeping gene Ubq (LOC_Os03g13170) as an internal control. Three biological replicates were performed for each experiment.