sCSF1R increased the resistance of cells to the chemotherapeutic
drug cytarabine
Transcriptome studies have shown that a few monocytes are potential
precursors in the circulation for LCH11,12. To
evaluate the effect of sCSF1R on the chemosensitivity of LCH cells, we
infected THP-1 cells with a BRAF -V600E overexpression lentivirus,
which resulted in a significant increase in ERK1/2 phosphorylation
(Supplementary Figure S5A). MTS experiments showed significantly
elevated IC50 values after incubation with sCSF1R compared to those of
the control (IC50: 4.6 vs . 2.3 μmol/L, P = 0.02;
Supplementary Figure S5B), indicating increased resistance to cytarabine
(Ara-C) resulting from sCSF1R treatment.