**Table 3 here**

3.3 CCRD Results for Oil-Free Seeds

In Table 4 are presented the experimental data using oil-free seeds as the enzymatic catalyst, where an enzymatic hydrolysis yield variation of 28.27 to 69.30% in the investigated conditions can be observed.
Through the Pareto graph (Figure 4 ) it is found that all variables were significant (95% level of significance), including the interactions between the variables, with the highest effect variable being the catalyst/substrate ratio, followed by the linear effect of the buffer/oil ratio and the interaction between the two variables. The RSM equation is given by Equation (5) . The f-test was employed to evaluate the model, and the calculated f-value (18.06) was again higher than the critical f-value (3.68). Hence, the regression model is statistically valid, and satisfactorily represent the experimental data within the studied conditions range. The coefficient of determination (R2) of 0.96 establish the accuracy of the regression model with the experimental data.