**Table 3 here**
3.3 CCRD Results for Oil-Free
Seeds
In Table 4 are presented the experimental data using oil-free
seeds as the enzymatic catalyst, where an enzymatic hydrolysis yield
variation of 28.27 to 69.30% in the investigated conditions can be
observed.
Through the Pareto graph (Figure 4 ) it is found that all
variables were significant (95% level of significance), including the
interactions between the variables, with the highest effect variable
being the catalyst/substrate ratio, followed by the linear effect of the
buffer/oil ratio and the interaction between the two variables. The RSM
equation is given by Equation (5) . The f-test was employed to
evaluate the model, and the calculated f-value (18.06) was again higher
than the critical f-value (3.68). Hence, the regression model is
statistically valid, and satisfactorily represent the experimental data
within the studied conditions range. The coefficient of determination
(R2) of 0.96 establish the accuracy of the regression
model with the experimental data.