Statistical analyses
Logarithm of odds (LOD) scores for data of NMD02 were calculated using MLINK program of the Linkage 3.3 package.(Terwilliger & Ott, 1994) Only the participating branch was included for the purpose of LOD score calculations.
The siRNA experiments were independently performed twice, and for each experiment we had triplicates for each condition. The values in the graphs were calculated and presented as average ± standard error of the mean (SEM) of technical replicates. Significance of each siRNA-treated sample compared to scrambled control was calculated by unpaired T test, using GraphPad Prism 7.0 (GraphPad Software, Inc., La Jolla, CA, USA) using raw data for absorbance and qPCR.