TMEM251 localizes to Golgi complex and Plasma membrane
Online searches indicated that TMEM251 is likely to be localized to the Golgi complex in humans and mice. GFP-fused wild-type TMEM251 was observed to localize to plasma membrane and strongly co-localized with a marker of Golgi complex, Giantin, in transiently transfected Human Osteosarcoma cells (Figure 4A). GFP-fused p.Arg45Trp TMEM251 also localized to Golgi complex and cell membrane (Figure 4B). In contrast, both wild-type and mutant proteins did not co-localize with markers of Endoplasmic reticulum (PDI) (Figure 4C-D) or lysosome (LAMP1) (not shown). The expression of GFP-fused p.Arg45Trp TMEM251 was markedly reduced and punctate when compared to that of the wild-type protein (Figure 4B and D).