cDNA synthesis
We extracted RNA from blood (human) or brain, liver and kidney (mouse)
using Tri Reagent (Thermo Fisher Scientific). The cDNA libraries were
prepared using RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher
Scientific) primed with a Poly T primer. TMEM251 cDNA was
amplified using gene specific primers (Table S3). The cDNA were cloned
by T/A cloning into pTZ57R/T vector (Thermo Fisher Scientific) and
sequence verified.