Statistical analyses
Logarithm of odds (LOD) scores for data of NMD02 were calculated using
MLINK program of the Linkage 3.3 package.(Terwilliger & Ott, 1994) Only
the participating branch was included for the purpose of LOD score
calculations.
The siRNA experiments were independently performed twice, and for each
experiment we had triplicates for each condition. The values in the
graphs were calculated and presented as average ± standard error of the
mean (SEM) of technical replicates. Significance of each siRNA-treated
sample compared to scrambled control was calculated by unpaired T test,
using GraphPad Prism 7.0 (GraphPad Software, Inc., La Jolla, CA, USA)
using raw data for absorbance and qPCR.