Statistical analysis:
Data normality was assessed by Kolmogorov-Smirnov test. Quantitative comparisons without a normal distribution were carried out using Mann-Whitney and Kruskal Wallis-tests. Comparisons of qualitative variables were performed using the X2 test. Receiver operating characteristic curves (ROC curves) were performed for getting cut-off point to indicate positive results. P-values less than 0.05 were considered statistically significant.