1.3.2. Preparation of ethanolic extract
Cold maceration was used to extrate 1200g of powder in 14 L of ethanol for 48 h. The mixture was filtered and the filtrate was concentrated in a rotary evaporator (BUCHI). The concentrated extract was dried using ventilated drying oven (MANESTY- PETRIE) to obtain 34.6g of dry powdered extract.