1.3.3. Preparation of hydroethanolic extract
Hydroethanolic extract was prepared by maceration of powder (2400g) into
ethanol /water (70:30) for 48h. The filtered macerate was concentrated
using rotary evaporator (BUCHI) and dried out using ventilated drying
oven (MANESTY- PETRIE) to obtain 263.09g of dry extract.