1.1. Chemicals
Copper sulfate (CuSO4), ethylenediaminetetra acetic acid (EDTA), trichloroacetic acid (TCA), thiobarbituric acid (TBA), solid potassium bromide (KBr), sodium chloride (NaCl), sulfuric acid, sodium phosphate, ammonium molybdate, sodium nitroprusside, dibasic sodium phospate (Na2HPO4) (for PBS preparation), monobasic sodium phosphate (NaH2PO4) (for PBS preparation), sulphanilamide, phosphoric acid (H3PO4),napthylethylenediamine dihydrochloride, hydrogen peroxide (H2O2), ascorbic acid, methanol, sulphuric acid, chloroform, ethanol, diethyl ether, n-butanol, were facilited by the Pharmacology Laboratory of the MM college of Pharmacy, Mullana, Ambala,India where different tests were conducted.
Plant material
The fresh leaves of P. glandulosus were collected in Ngaoundere (Cameroon). The plant was identified at the National Herbarium of Cameroon, where the voucher specimen was deposited (41168-HCN). The fresh leaves collected from their natural environment were washed with distilled water three times, shade dried and powdered.
1.3. Extraction and fractionation of plant materials .