1.1. Chemicals
Copper sulfate (CuSO4), ethylenediaminetetra acetic acid
(EDTA), trichloroacetic acid (TCA), thiobarbituric acid (TBA), solid
potassium bromide (KBr), sodium chloride (NaCl), sulfuric acid, sodium
phosphate, ammonium molybdate, sodium nitroprusside, dibasic sodium
phospate (Na2HPO4) (for PBS preparation), monobasic
sodium phosphate (NaH2PO4) (for PBS preparation),
sulphanilamide, phosphoric acid
(H3PO4),napthylethylenediamine dihydrochloride, hydrogen peroxide
(H2O2), ascorbic acid, methanol,
sulphuric acid, chloroform, ethanol, diethyl ether, n-butanol, were
facilited by the Pharmacology Laboratory of the MM college of Pharmacy,
Mullana, Ambala,India where different tests were conducted.
Plant material
The fresh leaves of P. glandulosus were collected in Ngaoundere
(Cameroon). The plant was identified at the National Herbarium of
Cameroon, where the voucher specimen was deposited (41168-HCN). The
fresh leaves collected from their natural environment were washed with
distilled water three times, shade dried and powdered.
1.3. Extraction and fractionation of plant materials .