Figure 1. PDCoV isolation in PK-15 cells and geographical map
indicating locations where the outbreak was reported
Following PDCoV detection, intestinal content was filtered, and
TPCK-trypsin treated for isolation in cell lines known to be permissible
for PDCoV replication. Following 5 days of inoculation, no cytopathic
effect (cpe) was evidenced under light microscopy in both cell lines. A
second passage evidenced cpe after 24 h, with initial cell rounding and
monolayer disruption in PK-15 cells but absent in Vero cells. Following
48 h of second passage, cpe was evidenced in almost 70 % of cell
monolayer, characterized by cell rounding, cell detaching, pyknosis in
PK-15 cell lines but no cytopathic effect was detected in Vero cell
lines (A). Moreover, an immunofluorescence test with a PDCoV
nucleoprotein monoclonal antibody confirmed viral replication in PK-15
infected cells (B). A representative map of South America (blue)
highlighting Peru (orange) is shown in the upper right side.
Additionally, a geographical map of San Martin department (yellow)
located in the north of Peru (light green) is represented below (C).