2.1. Samples and RNA extraction:
In November 2019, a report of an enteric clinical disease outbreak in a
farm located in San Martin, Peru (see Figure 1C) was made by a local
veterinary professional to the National animal health authorities
(SENASA). The report indicated high morbidity in young piglets.
Intestinal samples were submitted by the farm professionals to the
Laboratory of Virology in the Faculty of Veterinary Medicine at the
Universidad Nacional Mayor de San Marcos in Lima, Peru; for testing and
detection of PEDV, TGEV and PDCoV by qRT-PCR. Intestinal content (1 ml)
was processed accordingly. In short, samples were diluted in 5ml of
phosphate buffer solution (PBS) and centrifuged at 250 g for 5 minutes.
Following centrifugation, 1 ml of supernatant was processed for RNA
extraction using the QIAmp viral RNA mini kit (Qiagen), following
manufacturer’s specifications.