Figure 4. Phylogenetic analysis of S gene reveals that Peruvian
PDCoV strain has a close relationship with a Mexican strain within the
North American phylogroup.
The evolutionary history was inferred by using the Maximum Likelihood
method and General Time Reversible model. The percentage of trees in
which the associated taxa clustered together is shown next to the
branches. A discrete Gamma distribution was used to model evolutionary
rate differences among. The tree is drawn to scale, with branch lengths
measured in the number of substitutions per site. This analysis involved
28 nucleotide sequences. Evolutionary analyses were conducted in MEGA X
(A). Schematic representation of the Porcine Deltacoronavirus S protein
highlighting the amino acid substitutions found in the present study.
Black arrows represent the site of these substitutions. A multiple amino
acid sequence alignment of S protein was performed using ClustalW in
MEGA X. Eight amino acid modifications were detected within the Peruvian
sequence compared to others (B).