Cell culture and IL-13 stimulation
The hNECs were differentiated from human nasal epithelial
stem/progenitor cells (hNESPCs) isolated from IT of healthy subjects
(n=9). The hNESPCs were transferred to an ALI system to form a
pseudostratified layer within 4 weeks. Methods for culturing hNECs were
described in previous paper23. IL-13 (10ng/ml, R&D
System, Minneapolis, MN, USA) was added in the medium on the first of
ALI and medium with IL-13 were replenished every 2 days for 21 days
until they were fully differentiated.