Immunofluorescence (IF) staining
IF staining was performed for paraffin sections of nasal tissue,
transwell membranes of ALI culture and cytospin clinical samples. Sample
sections were blocked with 10% normal goat serum, then incubated with a
primary antibody (Table E1 ) overnight at 4℃, followed by 60 min
incubation of Alexa Fluor 488- or Alexa Fluor 594-conjugated secondary
antibodies in the dark at room temperature. Mounting medium with
4’6-diamidino-2-phenylindole (DAPI) (Life Technologies, Carlsbad, CA)
was used to stain cell nucleus.