Infection and quantification of RV
Fully differentiated hNECs with and without IL-13 treatment were
inoculated with RV16 (ATCC VR-283®) at a high dosage of MOI 10 and
incubated at 33 °C for 1 h. Apical wash and RV-infected and uninfected
hNECs were harvested at 24 h post-infection. Plaque assay was performed
as previously described24. The plaque-forming unit
(PFU) was calculated as follows: Number of plaques × dilution factor =
number of PFU per 100 µl.